Submission Type

Poster

Start Date

4-26-2023

Abstract

Our goal for this project is to verify that the neurod4 gene was indeed passed down to future generations of zebrafish from the original transgenic fish. In order to correctly genotype the zebrafish used for regenerative research in the Bailey lab, DNA extraction and isolation, along with PCR analysis and gel electrophoresis are used. We are specifically amplifying the neurod4 gene responsible for regulation of neuronal development and differentiation. Using various polymerases purchased through the Geneseo TRAC Grant and multiple methods of experimentation, we have been attempting to amplify the specific region of the zebrafish genome that contains this gene which would allow us to genotype the fish correctly. These correctly genotyped fish could then be used for further research in regenerative and developmental biology by students in the Bailey lab.

Comments

Sponsored by Travis Bailey

Share

COinS
 
Apr 26th, 12:00 AM

035 - PCR Analysis of Transgenic Zebrafish Furthering Regenerative Research and Development

Our goal for this project is to verify that the neurod4 gene was indeed passed down to future generations of zebrafish from the original transgenic fish. In order to correctly genotype the zebrafish used for regenerative research in the Bailey lab, DNA extraction and isolation, along with PCR analysis and gel electrophoresis are used. We are specifically amplifying the neurod4 gene responsible for regulation of neuronal development and differentiation. Using various polymerases purchased through the Geneseo TRAC Grant and multiple methods of experimentation, we have been attempting to amplify the specific region of the zebrafish genome that contains this gene which would allow us to genotype the fish correctly. These correctly genotyped fish could then be used for further research in regenerative and developmental biology by students in the Bailey lab.

 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.