The search for alternative ways to create a more sustainable and environmentally friendly source of energy, other than fossil fuels, has been ongoing for many years. Particularly, the production of biofuels has become of interest. One effort has been through the creation of first-generation biofuels that curb greenhouse gas emissions, unlike fossil fuels. However, first generation biofuels lead to the increase in food prices which negatively impacts developing countries, as they use food sources to produce biofuel. To mitigate this issue, second generation biofuels are considered as a better alternative. Instead of hindering the food supply, second generation biofuels aim to not affect the food supply, by using non-human food sources. Furthermore, second generation biofuels are relatively inexpensive. The use of rice husks is of interest, as they can satisfy around one fifth of the global biofuel demand for a ten percent gasohol fuel blend.Therefore, glucose quantification is a necessary step in confirming the effectiveness of biofuels derivedfrom rice husks. Two methods that allow for the concentration of glucose to be quantified are dinitrosalicylic acid (DNS) analysis and the standard addition method. Through the analysis of each method, it can be determined if DNS analysis and the standard addition method are reliable glucose quantification procedures.
Vogler, Dineen and Gikonyo, Barnabas, "Bioethanol Production: Glucose Testing and Quantification Using DNS Analysis and Addition Analytical Methods" (2021). McNair Scholars Program. 7.