362— The Effects of Epigenetic Modifiers on the Expression of HLA-ABC in a Melanoma Cancer Cell Line
Submission Type
Poster
Start Date
April 2021
Abstract
Melanoma is widely known as a malignant and detrimental cancer that develops from the pigment-producing cells known as melanocytes. The human leukocyte antigen (HLA) is an important signaling molecule encoded by the major histocompatibility complex (MHC) that allows the immune system to detect and destroy cancer cells. I conducted various experiments using the epigenetic modifier drugs 5-Azacytidine and Vorinostat as well as the cytokine known as gamma interferon. 5-Azacytidine is a methylation inhibitor, and Vorinostat is a histone deacetylase inhibitor. Interferon gamma influences downstream gene expression. The purpose of using these drugs was to see if there was an effect on HLA expression of the 435 Melanoma cell line. Numerous flow cytometry experiments were conducted to determine if these drugs had any effect on the HLA expression on the 435- cell line. The data suggests that both Vorinostat and 5-Azacytidine increased HLA expression on the 435-cell line. Future experiments will be conducted to test whether the addition of gamma interferon to these drugs will result in an additional, synergistic, or antagonistic effect on the HLA expression.
Recommended Citation
Bussey, Hanna, "362— The Effects of Epigenetic Modifiers on the Expression of HLA-ABC in a Melanoma Cancer Cell Line" (2021). GREAT Day Posters. 2.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2021/posters-2021/2
362— The Effects of Epigenetic Modifiers on the Expression of HLA-ABC in a Melanoma Cancer Cell Line
Melanoma is widely known as a malignant and detrimental cancer that develops from the pigment-producing cells known as melanocytes. The human leukocyte antigen (HLA) is an important signaling molecule encoded by the major histocompatibility complex (MHC) that allows the immune system to detect and destroy cancer cells. I conducted various experiments using the epigenetic modifier drugs 5-Azacytidine and Vorinostat as well as the cytokine known as gamma interferon. 5-Azacytidine is a methylation inhibitor, and Vorinostat is a histone deacetylase inhibitor. Interferon gamma influences downstream gene expression. The purpose of using these drugs was to see if there was an effect on HLA expression of the 435 Melanoma cell line. Numerous flow cytometry experiments were conducted to determine if these drugs had any effect on the HLA expression on the 435- cell line. The data suggests that both Vorinostat and 5-Azacytidine increased HLA expression on the 435-cell line. Future experiments will be conducted to test whether the addition of gamma interferon to these drugs will result in an additional, synergistic, or antagonistic effect on the HLA expression.
Comments
Sponsored by Robert O'Donnell