Submission Type
Poster
Start Date
4-21-2022
Abstract
Zebrafish embryos that are homozygous for gef mutant alleles experience a small-eye phenotype and are hypothesized to have decreased levels of the Chaf1b protein. This could be detected by anti-Chaf1b antibodies in a western blot. This western blot will be performed using Chaf1b morpholino 3 dpf embryos, wild type 3 dpf embryos, and gef mutant embryos 3 dpf. Embryos inoculated with Chaf1b morpholino serve as a negative control. This was then compared to the positive control of wild type zebrafish embryos, whose western blot was expected to have normal levels of Chaf1b. Proteins isolated from a gef mutant were then be analyzed and compared to the control proteins to determine the levels of the Chaf1b protein in the mutant. Future research can expand on the in vitro role of Chaf 1b in multicellular organisms and this protein’s significance in regeneration.
Recommended Citation
Ulrich, Laura; Donlon, Brianna; Choku, Choeyang; and Maloney, Jacqueline, "199 -- Chaf1b Protein in gef Mutant Zebrafish" (2022). GREAT Day Posters. 65.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2022/posters-2022/65
199 -- Chaf1b Protein in gef Mutant Zebrafish
Zebrafish embryos that are homozygous for gef mutant alleles experience a small-eye phenotype and are hypothesized to have decreased levels of the Chaf1b protein. This could be detected by anti-Chaf1b antibodies in a western blot. This western blot will be performed using Chaf1b morpholino 3 dpf embryos, wild type 3 dpf embryos, and gef mutant embryos 3 dpf. Embryos inoculated with Chaf1b morpholino serve as a negative control. This was then compared to the positive control of wild type zebrafish embryos, whose western blot was expected to have normal levels of Chaf1b. Proteins isolated from a gef mutant were then be analyzed and compared to the control proteins to determine the levels of the Chaf1b protein in the mutant. Future research can expand on the in vitro role of Chaf 1b in multicellular organisms and this protein’s significance in regeneration.
Comments
Sponsored by Travis Bailey