Submission Type

Poster

Start Date

4-21-2022

Abstract

Zebrafish embryos that are homozygous for gef mutant alleles experience a small-eye phenotype and are hypothesized to have decreased levels of the Chaf1b protein. This could be detected by anti-Chaf1b antibodies in a western blot. This western blot will be performed using Chaf1b morpholino 3 dpf embryos, wild type 3 dpf embryos, and gef mutant embryos 3 dpf. Embryos inoculated with Chaf1b morpholino serve as a negative control. This was then compared to the positive control of wild type zebrafish embryos, whose western blot was expected to have normal levels of Chaf1b. Proteins isolated from a gef mutant were then be analyzed and compared to the control proteins to determine the levels of the Chaf1b protein in the mutant. Future research can expand on the in vitro role of Chaf 1b in multicellular organisms and this protein’s significance in regeneration.

Comments

Sponsored by Travis Bailey

COinS
 
Apr 21st, 12:00 AM

199 -- Chaf1b Protein in gef Mutant Zebrafish

Zebrafish embryos that are homozygous for gef mutant alleles experience a small-eye phenotype and are hypothesized to have decreased levels of the Chaf1b protein. This could be detected by anti-Chaf1b antibodies in a western blot. This western blot will be performed using Chaf1b morpholino 3 dpf embryos, wild type 3 dpf embryos, and gef mutant embryos 3 dpf. Embryos inoculated with Chaf1b morpholino serve as a negative control. This was then compared to the positive control of wild type zebrafish embryos, whose western blot was expected to have normal levels of Chaf1b. Proteins isolated from a gef mutant were then be analyzed and compared to the control proteins to determine the levels of the Chaf1b protein in the mutant. Future research can expand on the in vitro role of Chaf 1b in multicellular organisms and this protein’s significance in regeneration.

 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.