Submission Type
Poster
Start Date
4-26-2023
Abstract
In our research, we aimed to examine the behavior of the spike protein (s-protein) of SARS-CoV-2, the virus responsible for COVID-19, on nano-gold colloid in the presence of ACE2 (angiotensin converting enzyme 2), which facilitates infection by binding to the s-protein. To do this, we coated gold colloid particles with the s-protein, ranging in size from 10 nm to 100 nm in diameter. We found that the Surface Plasmon Resonance (SPR) band was sensitive to aggregation and exhibited a red shift at pH ~3. We monitored the peak of the SPR band alternately at pH ~3 and pH ~11 over 10 cycles. For s-protein coated particles with a diameter greater than 30 nm, we observed enhanced reversible aggregation in the presence of ACE2. Currently, we are investigating the temperature dependence of this reversible aggregation and collecting and analyzing Surface Enhanced Raman Spectrum (SERS) data.
Recommended Citation
Kieft, Madison, "251 - Nano-size dependence of reversible aggregation and effect of ACE2 by SARS-CoV-2 spike-protein coated gold colloid" (2023). GREAT Day Posters. 13.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2023/posters-2023/13
251 - Nano-size dependence of reversible aggregation and effect of ACE2 by SARS-CoV-2 spike-protein coated gold colloid
In our research, we aimed to examine the behavior of the spike protein (s-protein) of SARS-CoV-2, the virus responsible for COVID-19, on nano-gold colloid in the presence of ACE2 (angiotensin converting enzyme 2), which facilitates infection by binding to the s-protein. To do this, we coated gold colloid particles with the s-protein, ranging in size from 10 nm to 100 nm in diameter. We found that the Surface Plasmon Resonance (SPR) band was sensitive to aggregation and exhibited a red shift at pH ~3. We monitored the peak of the SPR band alternately at pH ~3 and pH ~11 over 10 cycles. For s-protein coated particles with a diameter greater than 30 nm, we observed enhanced reversible aggregation in the presence of ACE2. Currently, we are investigating the temperature dependence of this reversible aggregation and collecting and analyzing Surface Enhanced Raman Spectrum (SERS) data.
Comments
Sponsored by Kazushige Yokoyama