Submission Type
Poster
Abstract
Neurod4 is a protein coding gene, also known as neurogenic differentiation 4. This gene prevails within the nervous system, specifically expressed in the brain. Neurod4 helps control any extreme, rapid growth of the photoreceptors in Zebrafish retina. Zebrafish have a similar, yet not identical gene to humans, thus, research done with zebrafish can correlate to how Neurod4 impacts human retinal neuronal development. Zebrafish used in this study are transgenic organisms, containing the Tg (neurod4: GFP). Transgenes are sections of genetic material used for genome modification of specific organisms. Zebrafish were genetically manipulated to express Neurod4. It is assumed that the transgene will share similar activity as Neurod4 gene, the transgene displays the same transcript expression as the endogenous gene. For the transgene to be expressed, it is necessary to polymerase the RNA strand. If this were to work on Zebrafish, we could then do a similar experiment on humans.
Recommended Citation
Flick, JULIANA, "033-Fluorescence In Situ Hybridization of Neurod4 Gene in Zebrafish" (2024). GREAT Day Posters. 11.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2024/posters-2024/11
033-Fluorescence In Situ Hybridization of Neurod4 Gene in Zebrafish
Neurod4 is a protein coding gene, also known as neurogenic differentiation 4. This gene prevails within the nervous system, specifically expressed in the brain. Neurod4 helps control any extreme, rapid growth of the photoreceptors in Zebrafish retina. Zebrafish have a similar, yet not identical gene to humans, thus, research done with zebrafish can correlate to how Neurod4 impacts human retinal neuronal development. Zebrafish used in this study are transgenic organisms, containing the Tg (neurod4: GFP). Transgenes are sections of genetic material used for genome modification of specific organisms. Zebrafish were genetically manipulated to express Neurod4. It is assumed that the transgene will share similar activity as Neurod4 gene, the transgene displays the same transcript expression as the endogenous gene. For the transgene to be expressed, it is necessary to polymerase the RNA strand. If this were to work on Zebrafish, we could then do a similar experiment on humans.