Submission Type
Poster
Abstract
CM-272 is a DNA methyltransferase inhibitor. Previous work in the Militello laboratory indicates that CM-272 has novel antibacterial properties. Unexpectedly, we have determined CM-272 fluoresces blue. The purpose of this project is to discover the absorbance, excitation, emission properties of CM-272 in order to take advantage of CM-272 for fluorescence applications. For all tests, readings were taken at multiple CM-272 concentrations to determine which concentration yields the best results. We found that the concentration yielding best results was with 10% CM-272. On an absorbance spectrum, 2 peaks were found at around 300 and 350 nanometers. Next, a fluorescence spectrometer was used to detect the excitation and emission. We suspected the emission would be at about 450 nm since the compound fluoresces blue. The emission value is 380 nm and the excitation value found is about 310 nm and 360 nm. The values found from these experiments can be used in future experiments when tracking CM-272 and will help to determine the mechanism of antibacterial action.
Recommended Citation
Samsel, Kaitlyn, "127-Analyzing CM-272 Properties using a Nanospectrometer and a Fluorescence Spectrometer" (2024). GREAT Day Posters. 28.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2024/posters-2024/28
127-Analyzing CM-272 Properties using a Nanospectrometer and a Fluorescence Spectrometer
CM-272 is a DNA methyltransferase inhibitor. Previous work in the Militello laboratory indicates that CM-272 has novel antibacterial properties. Unexpectedly, we have determined CM-272 fluoresces blue. The purpose of this project is to discover the absorbance, excitation, emission properties of CM-272 in order to take advantage of CM-272 for fluorescence applications. For all tests, readings were taken at multiple CM-272 concentrations to determine which concentration yields the best results. We found that the concentration yielding best results was with 10% CM-272. On an absorbance spectrum, 2 peaks were found at around 300 and 350 nanometers. Next, a fluorescence spectrometer was used to detect the excitation and emission. We suspected the emission would be at about 450 nm since the compound fluoresces blue. The emission value is 380 nm and the excitation value found is about 310 nm and 360 nm. The values found from these experiments can be used in future experiments when tracking CM-272 and will help to determine the mechanism of antibacterial action.