Submission Type
Poster
Abstract
Meiotic drive violates the fundamental law of segregation, changing allele inheritance from 50% to 100% of the time. An extreme sex-ratio meiotic drive (SR) trait is an X-linked selfish genetic element (SGE) which causes the carrier males to produce mostly female offspring. The presence of this traithas been identified in two stalk-eyed fly species,Teleopsis whiteiandTeleopsis dalmanni. InT. dalmanni, there are hundreds of differentially expressed genes, with virtually all gene expression and sequence differences confined to the X chromosome; however, this does not appear to be the case inT. whiteiin which only a few meiotic drive-associated differences appear, some of which are on the autosomes. To pinpoint differences associated with drive, whole genome sequencing was done on 10 SR and 10 standard (ST)T. whiteimales. There is not a genome forT. whiteiso our sequencing reads are mapped to theT. dalmannigenome. We used the program ANGSD to identify single nucleotide polymorphisms (SNPs) in our sequencing data in order to identify differences between SR and ST males. We found that there was too much variation on the X chromosome indicated by many heterozygous SNPs which is not possible in nature. We used ngsParalog to identify sites on the genome that showed evidence of mismapping, possibly due to the different genome used. We will next use our filtered data to observe patterns of genetic variation across the autosomes and X chromosome and see what SNPs vary between SR and ST males.
Recommended Citation
Cookinham, Ariana, "346-Evolutionary Genomics of Meiotic Drive in T. whitei" (2024). GREAT Day Posters. 94.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2024/posters-2024/94
346-Evolutionary Genomics of Meiotic Drive in T. whitei
Meiotic drive violates the fundamental law of segregation, changing allele inheritance from 50% to 100% of the time. An extreme sex-ratio meiotic drive (SR) trait is an X-linked selfish genetic element (SGE) which causes the carrier males to produce mostly female offspring. The presence of this traithas been identified in two stalk-eyed fly species,Teleopsis whiteiandTeleopsis dalmanni. InT. dalmanni, there are hundreds of differentially expressed genes, with virtually all gene expression and sequence differences confined to the X chromosome; however, this does not appear to be the case inT. whiteiin which only a few meiotic drive-associated differences appear, some of which are on the autosomes. To pinpoint differences associated with drive, whole genome sequencing was done on 10 SR and 10 standard (ST)T. whiteimales. There is not a genome forT. whiteiso our sequencing reads are mapped to theT. dalmannigenome. We used the program ANGSD to identify single nucleotide polymorphisms (SNPs) in our sequencing data in order to identify differences between SR and ST males. We found that there was too much variation on the X chromosome indicated by many heterozygous SNPs which is not possible in nature. We used ngsParalog to identify sites on the genome that showed evidence of mismapping, possibly due to the different genome used. We will next use our filtered data to observe patterns of genetic variation across the autosomes and X chromosome and see what SNPs vary between SR and ST males.