Submission Type
Poster
Abstract
Neurospora crassa is a model filamentous fungus that is widely used in a variety of genetics research. It is a reproductively versatile organism, with the ability to reproduce both sexually and asexually. N. crassa leaves many questions unanswered in regards to its mating behaviors. Specifically, we focus on how the transcription factor fsd-1 regulates mating behaviors of this fungus. Expression of fsd-1 allows for successful mating whereas strains with a deletion of fsd-1 (Δfsd-1) are functionally sterile. The focus of this study is the isolation of RNA from a mutant strain of Neurospora crassa containing a variant of the fsd-1 gene that has a single mutation in a key amino acid of its DNA binding domain. We have confirmed that this mutant strain is sterile and does not undergo mating. RNA will be isolated using the Trizol method and then sent out for RNA Illumina Sequencing. These results will be compared to those of previous RNA sequencing results for both the wild type and deletion strains. The Galaxy platform will be used for RNAseq analysis and to further draw conclusions regarding the differences between the wild type, deletion, and mutant fsd-1 N. crassa strains.
Recommended Citation
Bahm, Sophia and Doerrer, Leila, "173 - Isolation & RNA Sequencing of an N. crassa fsd-1 DNA Binding Domain Mutant Strain" (2025). GREAT Day Posters. 44.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2025/posters-2025/44
173 - Isolation & RNA Sequencing of an N. crassa fsd-1 DNA Binding Domain Mutant Strain
Neurospora crassa is a model filamentous fungus that is widely used in a variety of genetics research. It is a reproductively versatile organism, with the ability to reproduce both sexually and asexually. N. crassa leaves many questions unanswered in regards to its mating behaviors. Specifically, we focus on how the transcription factor fsd-1 regulates mating behaviors of this fungus. Expression of fsd-1 allows for successful mating whereas strains with a deletion of fsd-1 (Δfsd-1) are functionally sterile. The focus of this study is the isolation of RNA from a mutant strain of Neurospora crassa containing a variant of the fsd-1 gene that has a single mutation in a key amino acid of its DNA binding domain. We have confirmed that this mutant strain is sterile and does not undergo mating. RNA will be isolated using the Trizol method and then sent out for RNA Illumina Sequencing. These results will be compared to those of previous RNA sequencing results for both the wild type and deletion strains. The Galaxy platform will be used for RNAseq analysis and to further draw conclusions regarding the differences between the wild type, deletion, and mutant fsd-1 N. crassa strains.
Comments
Sponsored by Betsy Hutchison