Submission Type
Poster
Abstract
The Sonic Hedgehog protein (Shh) is required in the cell cycle for development and regeneration in zebrafish eyes. This is because it controls how cells differentiate into specialized cell types. The gene, ptch2, is also important due to it being a component of the Shh signaling. The retina needs to replace photoreceptors, a specific cell type of the retina, after it is damaged by light. It is unknown which retinal cells produce Shh. To detect which cells that express the shh and ptch2 mRNA, we plan to perform in situ hybridization using antisense probes on regenerating retinal sections and compare this to normal retinas. This will be done by attaching a fluorescent molecule to antisense shh and ptch2 synthetically transcribed RNAs. We are currently in the process of making probes.
Recommended Citation
Snyder, Adam, "216 - Location of Sonic Hedgehog Signaling in the Regenerating Zebrafish Retina." (2025). GREAT Day Posters. 63.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2025/posters-2025/63
216 - Location of Sonic Hedgehog Signaling in the Regenerating Zebrafish Retina.
The Sonic Hedgehog protein (Shh) is required in the cell cycle for development and regeneration in zebrafish eyes. This is because it controls how cells differentiate into specialized cell types. The gene, ptch2, is also important due to it being a component of the Shh signaling. The retina needs to replace photoreceptors, a specific cell type of the retina, after it is damaged by light. It is unknown which retinal cells produce Shh. To detect which cells that express the shh and ptch2 mRNA, we plan to perform in situ hybridization using antisense probes on regenerating retinal sections and compare this to normal retinas. This will be done by attaching a fluorescent molecule to antisense shh and ptch2 synthetically transcribed RNAs. We are currently in the process of making probes.
Comments
Sponsored by Travis Bailey