Submission Type
Poster
Abstract
An organism's gut microbiome has been shown previously to be influenced by outside factors including diet. The effects of a ketogenic diet (KD), which is a low carbohydrate, high-fat diet, and a standard diet (SD) the gut microbial composition of mice was analyzed over a 44 day time course, in both wild type and mutant mice which displayed repetitive motor behaviors (RMB). The stool of the mouse was collected at various time points and microbial DNA was extracted via a Qiagen FecalPro Kit. The 16S DNA of the samples were then sequenced using Illumina technology. Taxonomic analysis was completed to determine any changes in the gut microbiome. Changes of the microbial composition were seen between the keto diet and the standard diet fed mice. No conclusion could be made in regard to differences of genotypes fed the keto diet. These results indicate diet does alter the microbial composition however further research is needed to elucidate any other potential mechanism influencing the gut microbiome.
Recommended Citation
French, Makayla and O'Reilly, Hanna, "022 - The Effect of Diet on the Microbial Composition of the Mouse Gut" (2025). GREAT Day Posters. 7.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2025/posters-2025/7
022 - The Effect of Diet on the Microbial Composition of the Mouse Gut
An organism's gut microbiome has been shown previously to be influenced by outside factors including diet. The effects of a ketogenic diet (KD), which is a low carbohydrate, high-fat diet, and a standard diet (SD) the gut microbial composition of mice was analyzed over a 44 day time course, in both wild type and mutant mice which displayed repetitive motor behaviors (RMB). The stool of the mouse was collected at various time points and microbial DNA was extracted via a Qiagen FecalPro Kit. The 16S DNA of the samples were then sequenced using Illumina technology. Taxonomic analysis was completed to determine any changes in the gut microbiome. Changes of the microbial composition were seen between the keto diet and the standard diet fed mice. No conclusion could be made in regard to differences of genotypes fed the keto diet. These results indicate diet does alter the microbial composition however further research is needed to elucidate any other potential mechanism influencing the gut microbiome.
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