Submission Type
Poster
Abstract
An entire section of the hippocampus of the Long Evans Cohen's Alzheimer’s disease, AD(+), rat model was examined utilizing the Surface Enhanced Raman Scattering (SERS) spectrum to study gold aggregates. An examination of normal rat, AD(-), tissues was also performed as a comparison and negative control. In the AD(+) rat hippocampal tissues, a limited quantity of larger gold colloid aggregates were identified. The SERS spectrum of each section in the hippocampus exhibited nearly identical spectral patterns in the region of Amide I, II, and III bands, as well as distinct spectral patterns in the 300 cm–1 and 1250 cm–1 region that previously could only be observed in AD(+) rat tissue. The amyloid fibril with a β-sheet conformation, formerly identified and reported in the AD mouse and human brain tissues, was observed to form with gold colloid aggregates. Gold colloid aggregates were found in the hippocampus brain section of AD(+) rats, with unique morphological characteristics when compared to hippocampus tissues from AD(-) rats. While there were no clear differences in spectral patterns to distinguish between sections of the hippocampus, the current study provided a definitive characterization to differentiate the hippocampal tissues in AD(+) and AD(-) rats.
Recommended Citation
Brzezinski, Victoria and Yokoyama, Kazushige, "230 - Examination of Gold Colloid Aggregate Formations within the Hippocampus of the Alzheimer’s Disease Rat" (2025). GREAT Day Posters. 70.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2025/posters-2025/70
230 - Examination of Gold Colloid Aggregate Formations within the Hippocampus of the Alzheimer’s Disease Rat
An entire section of the hippocampus of the Long Evans Cohen's Alzheimer’s disease, AD(+), rat model was examined utilizing the Surface Enhanced Raman Scattering (SERS) spectrum to study gold aggregates. An examination of normal rat, AD(-), tissues was also performed as a comparison and negative control. In the AD(+) rat hippocampal tissues, a limited quantity of larger gold colloid aggregates were identified. The SERS spectrum of each section in the hippocampus exhibited nearly identical spectral patterns in the region of Amide I, II, and III bands, as well as distinct spectral patterns in the 300 cm–1 and 1250 cm–1 region that previously could only be observed in AD(+) rat tissue. The amyloid fibril with a β-sheet conformation, formerly identified and reported in the AD mouse and human brain tissues, was observed to form with gold colloid aggregates. Gold colloid aggregates were found in the hippocampus brain section of AD(+) rats, with unique morphological characteristics when compared to hippocampus tissues from AD(-) rats. While there were no clear differences in spectral patterns to distinguish between sections of the hippocampus, the current study provided a definitive characterization to differentiate the hippocampal tissues in AD(+) and AD(-) rats.
Comments
Sponsored by Kazushige Yokoyama