Submission Type
Poster
Abstract
Neurod4, neurogenic differentiation 4, is a protein encoding gene that is crucial for neuronal development and is expressed in the nervous system specifically within the brain. The purpose of this gene is to regulate the extreme, rapid growth of photoreceptor progenitors in the retina of Zebrafish (Danio rerio). Due to the highly similar, although not identical, gene patterns in humans, Zebrafish act as an exceptional model organism to provide significant insights of the role of Neurod4 in human retinal and neuronal development. The transgenic nature of Zebrafish allows for genetic modification to express the Neurod4 gene in this model organism. This allows for control of gene expression and to efficiently observe the role that it has in this study. Fluorescence In Situ Hybridization (FISH) is a laboratory technique used to locate a particular DNA sequence. A small piece of DNA is tagged with a fluorescent dye, known as a probe, and is used to bind to its matching DNA sequence within a chromosome. This experiment is using isolated DNA from the Neurod4 transgene to develop an RNA probe to inject Zebrafish embryos. Through successful completion of this technique, a fluorescent glow is expected behind the retinas and along the spine. This will confirm the location of the Neurod4 gene as well as if the transgene shares the same function as the endogenous gene.
Recommended Citation
Allam, Sarah; Schnauder, Olivia; Conover, Lauren; and Sunderland, Samuel, "272 - Florescence In Situ Hybridization in Zebrafish with the Neurod4 Gene" (2025). GREAT Day Posters. 82.
https://knightscholar.geneseo.edu/great-day-symposium/great-day-2025/posters-2025/82
272 - Florescence In Situ Hybridization in Zebrafish with the Neurod4 Gene
Neurod4, neurogenic differentiation 4, is a protein encoding gene that is crucial for neuronal development and is expressed in the nervous system specifically within the brain. The purpose of this gene is to regulate the extreme, rapid growth of photoreceptor progenitors in the retina of Zebrafish (Danio rerio). Due to the highly similar, although not identical, gene patterns in humans, Zebrafish act as an exceptional model organism to provide significant insights of the role of Neurod4 in human retinal and neuronal development. The transgenic nature of Zebrafish allows for genetic modification to express the Neurod4 gene in this model organism. This allows for control of gene expression and to efficiently observe the role that it has in this study. Fluorescence In Situ Hybridization (FISH) is a laboratory technique used to locate a particular DNA sequence. A small piece of DNA is tagged with a fluorescent dye, known as a probe, and is used to bind to its matching DNA sequence within a chromosome. This experiment is using isolated DNA from the Neurod4 transgene to develop an RNA probe to inject Zebrafish embryos. Through successful completion of this technique, a fluorescent glow is expected behind the retinas and along the spine. This will confirm the location of the Neurod4 gene as well as if the transgene shares the same function as the endogenous gene.
Comments
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