Document Type
Poster
Publication Date
4-17-2019
Abstract
Neurospora crassa is a filamentous fungus that can undergo either asexual or sexual reproduction. Little is known about the signaling mechanisms that control reproduction in N. crassa, as compared to what is known about sexual reproduction of yeast (Saccharomyces cerevisiae). A key regulator of the sexual cycle in yeast is the transcription factor NDT80 of which N. crassa has a homolog called fsd-1. There are three different variants of the fsd-1 gene which differ by the position of the transcriptional start codon and their 5’ untranslated regions. qRT-PCR data has shown that the second transcript is most highly expressed. However, further experiments are needed to verify that the protein is expressed in N. crassa. Previous western blot data for FSD-1 was inconclusive due to the overall low abundance of the FSD-1 protein. We are currently in the process of constructing copies of the fsd-1 gene with an overexpression promoter and a GFP tag at the N-terminal end, which will be targeted to the native location in the genome. These strains will not only allow us to study the phenotype of overexpression strains, but they will also be a valuable resource for protein expression.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Recommended Citation
Cotten, Katherine; Hales, Margaux; and Hutchison, Elizabeth, "Characterization of the expression of female sexual development-1 (fsd-1) in the fungus Neurospora crassa" (2019). Papers, Posters, and Recordings. 24.
https://knightscholar.geneseo.edu/great-day-works/24