Document Type
Poster
Publication Date
4-17-2019
Abstract
The ESP protein affects the pathway by which secondary metabolites form in Brassica rapa. This pathway is known as the myrosinase-glucosinolate pathway. Myrosinase, an enzyme, cleaves a specific bond in gluconapin, a glucosinolate derived from methionine; this results in theformation of secondary metabolites that function to defend the plant against herbivory. When ESP is present, it acts as a coenzyme of myrosinase, meaning it influences product formation. We are interested in defining exactly how the ESP protein does this. In order to determine the answer to our question, we harvest ESP protein from transformed E. coli bacteria for use in analytical assays. Interestingly, the transformed E. coli do not presently produce ESP. We have identified a possible cause of this problem, and we are currently working to remedy it so that we may perform the aforementioned assays.
Creative Commons License
This work is licensed under a Creative Commons Attribution-No Derivative Works 4.0 License.
Recommended Citation
Ess, Madeline; Card, Olivia; and Waite, Christina, "ESP`s Effect on the Glucosinolate-Myrosinase Pathway in B.rapa" (2019). Papers, Posters, and Recordings. 17.
https://knightscholar.geneseo.edu/great-day-works/17